scientific visualization package origin 7.0 Search Results


cv-1  (ATCC)
99
ATCC cv-1
Cv 1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cv-1/product/ATCC
Average 99 stars, based on 1 article reviews
cv-1 - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
chemiluminescence imaging system  (VILBER GmbH)
99
VILBER GmbH chemiluminescence imaging system
Chemiluminescence Imaging System, supplied by VILBER GmbH, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chemiluminescence imaging system/product/VILBER GmbH
Average 99 stars, based on 1 article reviews
chemiluminescence imaging system - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
fluorescein coupled dextran  (Thermo Fisher)
90
Thermo Fisher fluorescein coupled dextran
Fluorescein Coupled Dextran, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescein coupled dextran/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
fluorescein coupled dextran - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
alexa 647 conjugated dextran  (Thermo Fisher)
86
Thermo Fisher alexa 647 conjugated dextran
a . Live imaging of HNF4A-YFP (Vanslambrouck et al., 2019 JASN ) kidney organoids on differentiation day 18 with Alexa 647-conjugated dextran uptake. Quantification of individual uptake events displayed in lower left, and insets detail HNF4A-YFP and dextran distinction. Scale bars: whole organoid-500 microns, insets-100 microns. b . Whole-mount immunofluorescence of day 18 control and proximal-biased kidney organoids following dextran uptake assay. Boxed region is magnified and split by individual channels. Scale bars: 10 microns. c . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoids on differentiation day 21, split by control and proximal-biased conditions. Boxed area magnifies HNF4A + proximal organoid nephron regions. Scale bars: 10 microns. d . Timeline schematic of kidney organoid injury strategy. e . Quantification of relative protein signal from HAVCR1 + regions in control and proximal-biased kidney organoid nephrons on day 21 following injury. Data are averaged from n = 3 independent and consistent imaging panels. SEM error bars are shown. Statistical significance is determined using Student’s t test. f . Quantification of HNF4A + proximal organoid nephron segments with HAVCR1 expression for each of the four conditions from (c). Positive segments are counted from a 20X widefield panel of 4 images per organoid, from n = 2 biological replicate organoids for each condition. SEM error bars are shown. Statistical significance is determined using Student’s t test. g . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoid nephrons on day 21. Dashed white line indicates an uninterrupted segment through the lumen of the organoid tubule. Scale bars: 10 microns. h . Quantification of continuous lumen segments from (h). Data are quantified from n = 4 organoids per condition, and SEM error bars are shown. Statistical significance is determined using Student’s t test. i . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. Yellow arrows denote cells of the HNF4A + organoid nephron tubule with low HNF4A protein expression and positive expression of γH2AX. White arrows indicate cells with high HNF4A, and low γH2AX detection. Split channels separate HNF4A and γH2AX for cisplatin-injured condition. Scale bars: 10 microns. j . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. White arrowheads indicate interstitial cells surrounding the tubules that are positive for SOX9. Scale bars: 10 microns. k-l . Quantification of HNF4A and SOX9 signal intensities from (j) for uninjured (k) and +cisplatin (l) samples. Intensities were quantified from individual cells of technical and biological duplicates (4 organoids per condition) and normalized using min-max normalization.
Alexa 647 Conjugated Dextran, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alexa 647 conjugated dextran/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
alexa 647 conjugated dextran - by Bioz Stars, 2026-03
86/100 stars
  Buy from Supplier
nylon cell strainer corning falcontm  (Corning Life Sciences)
90
Corning Life Sciences nylon cell strainer corning falcontm
a . Live imaging of HNF4A-YFP (Vanslambrouck et al., 2019 JASN ) kidney organoids on differentiation day 18 with Alexa 647-conjugated dextran uptake. Quantification of individual uptake events displayed in lower left, and insets detail HNF4A-YFP and dextran distinction. Scale bars: whole organoid-500 microns, insets-100 microns. b . Whole-mount immunofluorescence of day 18 control and proximal-biased kidney organoids following dextran uptake assay. Boxed region is magnified and split by individual channels. Scale bars: 10 microns. c . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoids on differentiation day 21, split by control and proximal-biased conditions. Boxed area magnifies HNF4A + proximal organoid nephron regions. Scale bars: 10 microns. d . Timeline schematic of kidney organoid injury strategy. e . Quantification of relative protein signal from HAVCR1 + regions in control and proximal-biased kidney organoid nephrons on day 21 following injury. Data are averaged from n = 3 independent and consistent imaging panels. SEM error bars are shown. Statistical significance is determined using Student’s t test. f . Quantification of HNF4A + proximal organoid nephron segments with HAVCR1 expression for each of the four conditions from (c). Positive segments are counted from a 20X widefield panel of 4 images per organoid, from n = 2 biological replicate organoids for each condition. SEM error bars are shown. Statistical significance is determined using Student’s t test. g . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoid nephrons on day 21. Dashed white line indicates an uninterrupted segment through the lumen of the organoid tubule. Scale bars: 10 microns. h . Quantification of continuous lumen segments from (h). Data are quantified from n = 4 organoids per condition, and SEM error bars are shown. Statistical significance is determined using Student’s t test. i . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. Yellow arrows denote cells of the HNF4A + organoid nephron tubule with low HNF4A protein expression and positive expression of γH2AX. White arrows indicate cells with high HNF4A, and low γH2AX detection. Split channels separate HNF4A and γH2AX for cisplatin-injured condition. Scale bars: 10 microns. j . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. White arrowheads indicate interstitial cells surrounding the tubules that are positive for SOX9. Scale bars: 10 microns. k-l . Quantification of HNF4A and SOX9 signal intensities from (j) for uninjured (k) and +cisplatin (l) samples. Intensities were quantified from individual cells of technical and biological duplicates (4 organoids per condition) and normalized using min-max normalization.
Nylon Cell Strainer Corning Falcontm, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nylon cell strainer corning falcontm/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
nylon cell strainer corning falcontm - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
jasco cd spectrophotometer  (JASCO Inc)
99
JASCO Inc jasco cd spectrophotometer
a . Live imaging of HNF4A-YFP (Vanslambrouck et al., 2019 JASN ) kidney organoids on differentiation day 18 with Alexa 647-conjugated dextran uptake. Quantification of individual uptake events displayed in lower left, and insets detail HNF4A-YFP and dextran distinction. Scale bars: whole organoid-500 microns, insets-100 microns. b . Whole-mount immunofluorescence of day 18 control and proximal-biased kidney organoids following dextran uptake assay. Boxed region is magnified and split by individual channels. Scale bars: 10 microns. c . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoids on differentiation day 21, split by control and proximal-biased conditions. Boxed area magnifies HNF4A + proximal organoid nephron regions. Scale bars: 10 microns. d . Timeline schematic of kidney organoid injury strategy. e . Quantification of relative protein signal from HAVCR1 + regions in control and proximal-biased kidney organoid nephrons on day 21 following injury. Data are averaged from n = 3 independent and consistent imaging panels. SEM error bars are shown. Statistical significance is determined using Student’s t test. f . Quantification of HNF4A + proximal organoid nephron segments with HAVCR1 expression for each of the four conditions from (c). Positive segments are counted from a 20X widefield panel of 4 images per organoid, from n = 2 biological replicate organoids for each condition. SEM error bars are shown. Statistical significance is determined using Student’s t test. g . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoid nephrons on day 21. Dashed white line indicates an uninterrupted segment through the lumen of the organoid tubule. Scale bars: 10 microns. h . Quantification of continuous lumen segments from (h). Data are quantified from n = 4 organoids per condition, and SEM error bars are shown. Statistical significance is determined using Student’s t test. i . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. Yellow arrows denote cells of the HNF4A + organoid nephron tubule with low HNF4A protein expression and positive expression of γH2AX. White arrows indicate cells with high HNF4A, and low γH2AX detection. Split channels separate HNF4A and γH2AX for cisplatin-injured condition. Scale bars: 10 microns. j . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. White arrowheads indicate interstitial cells surrounding the tubules that are positive for SOX9. Scale bars: 10 microns. k-l . Quantification of HNF4A and SOX9 signal intensities from (j) for uninjured (k) and +cisplatin (l) samples. Intensities were quantified from individual cells of technical and biological duplicates (4 organoids per condition) and normalized using min-max normalization.
Jasco Cd Spectrophotometer, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/jasco cd spectrophotometer/product/JASCO Inc
Average 99 stars, based on 1 article reviews
jasco cd spectrophotometer - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
oleylamine  (Millipore)
90
Millipore oleylamine
a . Live imaging of HNF4A-YFP (Vanslambrouck et al., 2019 JASN ) kidney organoids on differentiation day 18 with Alexa 647-conjugated dextran uptake. Quantification of individual uptake events displayed in lower left, and insets detail HNF4A-YFP and dextran distinction. Scale bars: whole organoid-500 microns, insets-100 microns. b . Whole-mount immunofluorescence of day 18 control and proximal-biased kidney organoids following dextran uptake assay. Boxed region is magnified and split by individual channels. Scale bars: 10 microns. c . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoids on differentiation day 21, split by control and proximal-biased conditions. Boxed area magnifies HNF4A + proximal organoid nephron regions. Scale bars: 10 microns. d . Timeline schematic of kidney organoid injury strategy. e . Quantification of relative protein signal from HAVCR1 + regions in control and proximal-biased kidney organoid nephrons on day 21 following injury. Data are averaged from n = 3 independent and consistent imaging panels. SEM error bars are shown. Statistical significance is determined using Student’s t test. f . Quantification of HNF4A + proximal organoid nephron segments with HAVCR1 expression for each of the four conditions from (c). Positive segments are counted from a 20X widefield panel of 4 images per organoid, from n = 2 biological replicate organoids for each condition. SEM error bars are shown. Statistical significance is determined using Student’s t test. g . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoid nephrons on day 21. Dashed white line indicates an uninterrupted segment through the lumen of the organoid tubule. Scale bars: 10 microns. h . Quantification of continuous lumen segments from (h). Data are quantified from n = 4 organoids per condition, and SEM error bars are shown. Statistical significance is determined using Student’s t test. i . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. Yellow arrows denote cells of the HNF4A + organoid nephron tubule with low HNF4A protein expression and positive expression of γH2AX. White arrows indicate cells with high HNF4A, and low γH2AX detection. Split channels separate HNF4A and γH2AX for cisplatin-injured condition. Scale bars: 10 microns. j . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. White arrowheads indicate interstitial cells surrounding the tubules that are positive for SOX9. Scale bars: 10 microns. k-l . Quantification of HNF4A and SOX9 signal intensities from (j) for uninjured (k) and +cisplatin (l) samples. Intensities were quantified from individual cells of technical and biological duplicates (4 organoids per condition) and normalized using min-max normalization.
Oleylamine, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oleylamine/product/Millipore
Average 90 stars, based on 1 article reviews
oleylamine - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
ocelloscope  (Neu-tec Group)
94
Neu-tec Group ocelloscope
a . Live imaging of HNF4A-YFP (Vanslambrouck et al., 2019 JASN ) kidney organoids on differentiation day 18 with Alexa 647-conjugated dextran uptake. Quantification of individual uptake events displayed in lower left, and insets detail HNF4A-YFP and dextran distinction. Scale bars: whole organoid-500 microns, insets-100 microns. b . Whole-mount immunofluorescence of day 18 control and proximal-biased kidney organoids following dextran uptake assay. Boxed region is magnified and split by individual channels. Scale bars: 10 microns. c . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoids on differentiation day 21, split by control and proximal-biased conditions. Boxed area magnifies HNF4A + proximal organoid nephron regions. Scale bars: 10 microns. d . Timeline schematic of kidney organoid injury strategy. e . Quantification of relative protein signal from HAVCR1 + regions in control and proximal-biased kidney organoid nephrons on day 21 following injury. Data are averaged from n = 3 independent and consistent imaging panels. SEM error bars are shown. Statistical significance is determined using Student’s t test. f . Quantification of HNF4A + proximal organoid nephron segments with HAVCR1 expression for each of the four conditions from (c). Positive segments are counted from a 20X widefield panel of 4 images per organoid, from n = 2 biological replicate organoids for each condition. SEM error bars are shown. Statistical significance is determined using Student’s t test. g . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoid nephrons on day 21. Dashed white line indicates an uninterrupted segment through the lumen of the organoid tubule. Scale bars: 10 microns. h . Quantification of continuous lumen segments from (h). Data are quantified from n = 4 organoids per condition, and SEM error bars are shown. Statistical significance is determined using Student’s t test. i . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. Yellow arrows denote cells of the HNF4A + organoid nephron tubule with low HNF4A protein expression and positive expression of γH2AX. White arrows indicate cells with high HNF4A, and low γH2AX detection. Split channels separate HNF4A and γH2AX for cisplatin-injured condition. Scale bars: 10 microns. j . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. White arrowheads indicate interstitial cells surrounding the tubules that are positive for SOX9. Scale bars: 10 microns. k-l . Quantification of HNF4A and SOX9 signal intensities from (j) for uninjured (k) and +cisplatin (l) samples. Intensities were quantified from individual cells of technical and biological duplicates (4 organoids per condition) and normalized using min-max normalization.
Ocelloscope, supplied by Neu-tec Group, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ocelloscope/product/Neu-tec Group
Average 94 stars, based on 1 article reviews
ocelloscope - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
genius xe 70 laboratory gas generator  (Peak Scientific)
93
Peak Scientific genius xe 70 laboratory gas generator
a . Live imaging of HNF4A-YFP (Vanslambrouck et al., 2019 JASN ) kidney organoids on differentiation day 18 with Alexa 647-conjugated dextran uptake. Quantification of individual uptake events displayed in lower left, and insets detail HNF4A-YFP and dextran distinction. Scale bars: whole organoid-500 microns, insets-100 microns. b . Whole-mount immunofluorescence of day 18 control and proximal-biased kidney organoids following dextran uptake assay. Boxed region is magnified and split by individual channels. Scale bars: 10 microns. c . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoids on differentiation day 21, split by control and proximal-biased conditions. Boxed area magnifies HNF4A + proximal organoid nephron regions. Scale bars: 10 microns. d . Timeline schematic of kidney organoid injury strategy. e . Quantification of relative protein signal from HAVCR1 + regions in control and proximal-biased kidney organoid nephrons on day 21 following injury. Data are averaged from n = 3 independent and consistent imaging panels. SEM error bars are shown. Statistical significance is determined using Student’s t test. f . Quantification of HNF4A + proximal organoid nephron segments with HAVCR1 expression for each of the four conditions from (c). Positive segments are counted from a 20X widefield panel of 4 images per organoid, from n = 2 biological replicate organoids for each condition. SEM error bars are shown. Statistical significance is determined using Student’s t test. g . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoid nephrons on day 21. Dashed white line indicates an uninterrupted segment through the lumen of the organoid tubule. Scale bars: 10 microns. h . Quantification of continuous lumen segments from (h). Data are quantified from n = 4 organoids per condition, and SEM error bars are shown. Statistical significance is determined using Student’s t test. i . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. Yellow arrows denote cells of the HNF4A + organoid nephron tubule with low HNF4A protein expression and positive expression of γH2AX. White arrows indicate cells with high HNF4A, and low γH2AX detection. Split channels separate HNF4A and γH2AX for cisplatin-injured condition. Scale bars: 10 microns. j . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. White arrowheads indicate interstitial cells surrounding the tubules that are positive for SOX9. Scale bars: 10 microns. k-l . Quantification of HNF4A and SOX9 signal intensities from (j) for uninjured (k) and +cisplatin (l) samples. Intensities were quantified from individual cells of technical and biological duplicates (4 organoids per condition) and normalized using min-max normalization.
Genius Xe 70 Laboratory Gas Generator, supplied by Peak Scientific, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genius xe 70 laboratory gas generator/product/Peak Scientific
Average 93 stars, based on 1 article reviews
genius xe 70 laboratory gas generator - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
rf33.70  (New England Biolabs)
98
New England Biolabs rf33.70
a . Live imaging of HNF4A-YFP (Vanslambrouck et al., 2019 JASN ) kidney organoids on differentiation day 18 with Alexa 647-conjugated dextran uptake. Quantification of individual uptake events displayed in lower left, and insets detail HNF4A-YFP and dextran distinction. Scale bars: whole organoid-500 microns, insets-100 microns. b . Whole-mount immunofluorescence of day 18 control and proximal-biased kidney organoids following dextran uptake assay. Boxed region is magnified and split by individual channels. Scale bars: 10 microns. c . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoids on differentiation day 21, split by control and proximal-biased conditions. Boxed area magnifies HNF4A + proximal organoid nephron regions. Scale bars: 10 microns. d . Timeline schematic of kidney organoid injury strategy. e . Quantification of relative protein signal from HAVCR1 + regions in control and proximal-biased kidney organoid nephrons on day 21 following injury. Data are averaged from n = 3 independent and consistent imaging panels. SEM error bars are shown. Statistical significance is determined using Student’s t test. f . Quantification of HNF4A + proximal organoid nephron segments with HAVCR1 expression for each of the four conditions from (c). Positive segments are counted from a 20X widefield panel of 4 images per organoid, from n = 2 biological replicate organoids for each condition. SEM error bars are shown. Statistical significance is determined using Student’s t test. g . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoid nephrons on day 21. Dashed white line indicates an uninterrupted segment through the lumen of the organoid tubule. Scale bars: 10 microns. h . Quantification of continuous lumen segments from (h). Data are quantified from n = 4 organoids per condition, and SEM error bars are shown. Statistical significance is determined using Student’s t test. i . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. Yellow arrows denote cells of the HNF4A + organoid nephron tubule with low HNF4A protein expression and positive expression of γH2AX. White arrows indicate cells with high HNF4A, and low γH2AX detection. Split channels separate HNF4A and γH2AX for cisplatin-injured condition. Scale bars: 10 microns. j . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. White arrowheads indicate interstitial cells surrounding the tubules that are positive for SOX9. Scale bars: 10 microns. k-l . Quantification of HNF4A and SOX9 signal intensities from (j) for uninjured (k) and +cisplatin (l) samples. Intensities were quantified from individual cells of technical and biological duplicates (4 organoids per condition) and normalized using min-max normalization.
Rf33.70, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rf33.70/product/New England Biolabs
Average 98 stars, based on 1 article reviews
rf33.70 - by Bioz Stars, 2026-03
98/100 stars
  Buy from Supplier
ecgs  (Millipore)
90
Millipore ecgs
a . Live imaging of HNF4A-YFP (Vanslambrouck et al., 2019 JASN ) kidney organoids on differentiation day 18 with Alexa 647-conjugated dextran uptake. Quantification of individual uptake events displayed in lower left, and insets detail HNF4A-YFP and dextran distinction. Scale bars: whole organoid-500 microns, insets-100 microns. b . Whole-mount immunofluorescence of day 18 control and proximal-biased kidney organoids following dextran uptake assay. Boxed region is magnified and split by individual channels. Scale bars: 10 microns. c . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoids on differentiation day 21, split by control and proximal-biased conditions. Boxed area magnifies HNF4A + proximal organoid nephron regions. Scale bars: 10 microns. d . Timeline schematic of kidney organoid injury strategy. e . Quantification of relative protein signal from HAVCR1 + regions in control and proximal-biased kidney organoid nephrons on day 21 following injury. Data are averaged from n = 3 independent and consistent imaging panels. SEM error bars are shown. Statistical significance is determined using Student’s t test. f . Quantification of HNF4A + proximal organoid nephron segments with HAVCR1 expression for each of the four conditions from (c). Positive segments are counted from a 20X widefield panel of 4 images per organoid, from n = 2 biological replicate organoids for each condition. SEM error bars are shown. Statistical significance is determined using Student’s t test. g . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoid nephrons on day 21. Dashed white line indicates an uninterrupted segment through the lumen of the organoid tubule. Scale bars: 10 microns. h . Quantification of continuous lumen segments from (h). Data are quantified from n = 4 organoids per condition, and SEM error bars are shown. Statistical significance is determined using Student’s t test. i . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. Yellow arrows denote cells of the HNF4A + organoid nephron tubule with low HNF4A protein expression and positive expression of γH2AX. White arrows indicate cells with high HNF4A, and low γH2AX detection. Split channels separate HNF4A and γH2AX for cisplatin-injured condition. Scale bars: 10 microns. j . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. White arrowheads indicate interstitial cells surrounding the tubules that are positive for SOX9. Scale bars: 10 microns. k-l . Quantification of HNF4A and SOX9 signal intensities from (j) for uninjured (k) and +cisplatin (l) samples. Intensities were quantified from individual cells of technical and biological duplicates (4 organoids per condition) and normalized using min-max normalization.
Ecgs, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ecgs/product/Millipore
Average 90 stars, based on 1 article reviews
ecgs - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
biorex 70  (Bio-Rad)
90
Bio-Rad biorex 70
a . Live imaging of HNF4A-YFP (Vanslambrouck et al., 2019 JASN ) kidney organoids on differentiation day 18 with Alexa 647-conjugated dextran uptake. Quantification of individual uptake events displayed in lower left, and insets detail HNF4A-YFP and dextran distinction. Scale bars: whole organoid-500 microns, insets-100 microns. b . Whole-mount immunofluorescence of day 18 control and proximal-biased kidney organoids following dextran uptake assay. Boxed region is magnified and split by individual channels. Scale bars: 10 microns. c . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoids on differentiation day 21, split by control and proximal-biased conditions. Boxed area magnifies HNF4A + proximal organoid nephron regions. Scale bars: 10 microns. d . Timeline schematic of kidney organoid injury strategy. e . Quantification of relative protein signal from HAVCR1 + regions in control and proximal-biased kidney organoid nephrons on day 21 following injury. Data are averaged from n = 3 independent and consistent imaging panels. SEM error bars are shown. Statistical significance is determined using Student’s t test. f . Quantification of HNF4A + proximal organoid nephron segments with HAVCR1 expression for each of the four conditions from (c). Positive segments are counted from a 20X widefield panel of 4 images per organoid, from n = 2 biological replicate organoids for each condition. SEM error bars are shown. Statistical significance is determined using Student’s t test. g . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoid nephrons on day 21. Dashed white line indicates an uninterrupted segment through the lumen of the organoid tubule. Scale bars: 10 microns. h . Quantification of continuous lumen segments from (h). Data are quantified from n = 4 organoids per condition, and SEM error bars are shown. Statistical significance is determined using Student’s t test. i . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. Yellow arrows denote cells of the HNF4A + organoid nephron tubule with low HNF4A protein expression and positive expression of γH2AX. White arrows indicate cells with high HNF4A, and low γH2AX detection. Split channels separate HNF4A and γH2AX for cisplatin-injured condition. Scale bars: 10 microns. j . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. White arrowheads indicate interstitial cells surrounding the tubules that are positive for SOX9. Scale bars: 10 microns. k-l . Quantification of HNF4A and SOX9 signal intensities from (j) for uninjured (k) and +cisplatin (l) samples. Intensities were quantified from individual cells of technical and biological duplicates (4 organoids per condition) and normalized using min-max normalization.
Biorex 70, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biorex 70/product/Bio-Rad
Average 90 stars, based on 1 article reviews
biorex 70 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


a . Live imaging of HNF4A-YFP (Vanslambrouck et al., 2019 JASN ) kidney organoids on differentiation day 18 with Alexa 647-conjugated dextran uptake. Quantification of individual uptake events displayed in lower left, and insets detail HNF4A-YFP and dextran distinction. Scale bars: whole organoid-500 microns, insets-100 microns. b . Whole-mount immunofluorescence of day 18 control and proximal-biased kidney organoids following dextran uptake assay. Boxed region is magnified and split by individual channels. Scale bars: 10 microns. c . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoids on differentiation day 21, split by control and proximal-biased conditions. Boxed area magnifies HNF4A + proximal organoid nephron regions. Scale bars: 10 microns. d . Timeline schematic of kidney organoid injury strategy. e . Quantification of relative protein signal from HAVCR1 + regions in control and proximal-biased kidney organoid nephrons on day 21 following injury. Data are averaged from n = 3 independent and consistent imaging panels. SEM error bars are shown. Statistical significance is determined using Student’s t test. f . Quantification of HNF4A + proximal organoid nephron segments with HAVCR1 expression for each of the four conditions from (c). Positive segments are counted from a 20X widefield panel of 4 images per organoid, from n = 2 biological replicate organoids for each condition. SEM error bars are shown. Statistical significance is determined using Student’s t test. g . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoid nephrons on day 21. Dashed white line indicates an uninterrupted segment through the lumen of the organoid tubule. Scale bars: 10 microns. h . Quantification of continuous lumen segments from (h). Data are quantified from n = 4 organoids per condition, and SEM error bars are shown. Statistical significance is determined using Student’s t test. i . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. Yellow arrows denote cells of the HNF4A + organoid nephron tubule with low HNF4A protein expression and positive expression of γH2AX. White arrows indicate cells with high HNF4A, and low γH2AX detection. Split channels separate HNF4A and γH2AX for cisplatin-injured condition. Scale bars: 10 microns. j . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. White arrowheads indicate interstitial cells surrounding the tubules that are positive for SOX9. Scale bars: 10 microns. k-l . Quantification of HNF4A and SOX9 signal intensities from (j) for uninjured (k) and +cisplatin (l) samples. Intensities were quantified from individual cells of technical and biological duplicates (4 organoids per condition) and normalized using min-max normalization.

Journal: bioRxiv

Article Title: Stepwise developmental mimicry generates proximal-biased kidney organoids

doi: 10.1101/2024.06.28.601028

Figure Lengend Snippet: a . Live imaging of HNF4A-YFP (Vanslambrouck et al., 2019 JASN ) kidney organoids on differentiation day 18 with Alexa 647-conjugated dextran uptake. Quantification of individual uptake events displayed in lower left, and insets detail HNF4A-YFP and dextran distinction. Scale bars: whole organoid-500 microns, insets-100 microns. b . Whole-mount immunofluorescence of day 18 control and proximal-biased kidney organoids following dextran uptake assay. Boxed region is magnified and split by individual channels. Scale bars: 10 microns. c . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoids on differentiation day 21, split by control and proximal-biased conditions. Boxed area magnifies HNF4A + proximal organoid nephron regions. Scale bars: 10 microns. d . Timeline schematic of kidney organoid injury strategy. e . Quantification of relative protein signal from HAVCR1 + regions in control and proximal-biased kidney organoid nephrons on day 21 following injury. Data are averaged from n = 3 independent and consistent imaging panels. SEM error bars are shown. Statistical significance is determined using Student’s t test. f . Quantification of HNF4A + proximal organoid nephron segments with HAVCR1 expression for each of the four conditions from (c). Positive segments are counted from a 20X widefield panel of 4 images per organoid, from n = 2 biological replicate organoids for each condition. SEM error bars are shown. Statistical significance is determined using Student’s t test. g . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured kidney organoid nephrons on day 21. Dashed white line indicates an uninterrupted segment through the lumen of the organoid tubule. Scale bars: 10 microns. h . Quantification of continuous lumen segments from (h). Data are quantified from n = 4 organoids per condition, and SEM error bars are shown. Statistical significance is determined using Student’s t test. i . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. Yellow arrows denote cells of the HNF4A + organoid nephron tubule with low HNF4A protein expression and positive expression of γH2AX. White arrows indicate cells with high HNF4A, and low γH2AX detection. Split channels separate HNF4A and γH2AX for cisplatin-injured condition. Scale bars: 10 microns. j . Whole-mount immunofluorescence of uninjured and 5 µM cisplatin-injured proximal-biased kidney organoid nephrons on day 21. White arrowheads indicate interstitial cells surrounding the tubules that are positive for SOX9. Scale bars: 10 microns. k-l . Quantification of HNF4A and SOX9 signal intensities from (j) for uninjured (k) and +cisplatin (l) samples. Intensities were quantified from individual cells of technical and biological duplicates (4 organoids per condition) and normalized using min-max normalization.

Article Snippet: For the dextran uptake assay, organoids were treated and processed in the same way, except that TeSR-E6 was supplemented with Alexa-647-conjugated Dextran (Thermo Fisher Scientific, D22914).

Techniques: Imaging, Immunofluorescence, Control, Expressing